Открыт новый гражданский прект по изучению наиболее отдалённых от евразийских линий Y-ДНК

Originally posted by mouglley_gen at Открыт новый гражданский прект по изучению наиболее отдалённых от евразийских линий Y-ДНК

Оригинал:New A00 project

A new project dedicated to Y-haplogroup A00 is seeking funding. A00 is the most basal clade of the human Y-chromosome phylogeny and has so far been found in African Americans and Mbo people from Cameroon. This is how the funds raised will be used:

In this campaign, we're seeking the funds we need to launch our first phase of fieldwork in late August, 2013. Our overall research plan includes five field trips to sample peoples in different regions of the country. In the first trip, Matthew will travel to the remote rural villages where he was raised, in the mountainous, forested Nkongho-Mbo region, and collect at least 100 samples from three villages, which will then be sent to a lab to be screened for A00. More in-depth testing will focus on those A00 samples.

Planning and discussions are underway regarding which labs, probably more than one, will perform both SNP and STR testing on the samples. A full Y-chromosome sequence is on our wish list. This fundraising campaign, our first, will be limited to funding the DNA collection fieldwork. We'll be asking for your donations for lab testing in a separate campaign, in the near future. We need to limit the total amount of each fundraising campaign's goal to a modest amount we have some confidence of achieving within the set time, due to the all-or-nothing system used by Microryza.

 A00 is separated from the rest of mankind by >200 thousand years, but how closely related are different A00 chromosomes? It is necessary to have multiple samples to attempt to answer that question; I think it is quite probable that A00 is not limited to the area of Cameroon where it was discovered, but it probably makes sense to focus on it, since it is most likely to yield additional A00 samples given a sample size of 100.

A full Y-chromosome sequence of an A00 chromosome would also be very useful, since it would allow us to estimate its divergence from the A0-T remainder of mankind more securely.

The $2,500 they are seeking to launch their first field trip seems like a bargain to me.

Ещё:
Оригинал: New citizen science project to collect DNA samples from Cameroon

I wrote back in November last year of the citizen science discovery of haplogroup A00 the oldest and most deep-rooted branch of the human Y-chromosome tree which dates back around 338,000 years. The scientific paper resulting from this discovery "An African American Paternal Lineage Adds an Extremely Ancient Root to the Human Y Chromosome Phylogenetic Tree" was published in February of this year in the American Journal of Human Genetics.

Bonnie Schrack, the administrator of the Haplogroup A Project at Family Tree DNA who was instrumental in the discovery of haplogroup A00, is now launching an ambitious new citizen project. She is working in collaboration with Matthew Fomine Forka Leypey, a Cameroonian doctoral candidate at the University of Yaounde, on a project to collect many new DNA samples from Matthew’s own Mbo people, and others across Cameroon, in order to greatly expand our knowledge of A00.

I’m copying below with Bonnie’s permission the letter she submitted to our ISOGG Project Administrators’ mailing list as Bonnie can explain the project far more eloquently than I can:

Dear friends and fellow enthusiasts

I have an exciting announcement to share with you. Until now, we as genetic genealogists and researchers of deep ancestry have always been dependent on the field research carried out by professional, academic population geneticists, whose priorities and interests have been different from ours. They were the only ones with access to the grant funding necessary to finance such projects.

It's a new day now - the times they are a-changin'. "Crowdfunding" is one of the hottest new developments in the online world, and with good reason. Now, we the people can launch all kinds of projects, and we can decide what we want to support with our own funds.

Today we go live with our crowdfunding page for the first grassroots, citizen science organized project to collect DNA samples in the field, in Cameroon! We're using the Microryza website, which is devoted to crowdfunding science research. Here's the link:

http://microryza.com/projects/y-dna-a00-and-the-peoples-of-cameroon-in-search-of-the-homeland

OR

http://bit.ly/13Z3et8

Many of you heard about our discovery of the A00 haplogroup, the world's earliest-branching Y-chromosome lineage. It was found in a WTY of the Perrys, an African-American family with an extremely unusual and unique haplotype, and then we found a few haplotypes matching them from members of two African ethnic groups, the Mbo and the Bangwa, who are neighbors in Southwest Cameroon. A few tiny bits of Mbo DNA were shared with Dr. Michael Hammer, and sequenced by his lab and Thomas Krahn at FTDNA. The SNPs confirmed that they belonged to the same haplogroup as the Perry family.

Calculations by Dr. Fernando Mendez, and others in our community, have placed the branching age of this lineage at anywhere from 200,000 to 338,000 years ago - at the dawn of modern humans' emergence, or before. And so little is known about it!  How far does it extend from those few Mbo and Bangwa families, and can it be found in other peoples? Is A00 a remnant of the earliest, indigenous hunting and gathering peoples of Africa, and if so, when and where were they assimilated into other peoples, who are now settled farmers (though they still hunt)?

For the first time since A00 has been known to exist, a young Cameroonian scholar, Matthew Fomine Forka Leypey, a member of the Mbo ethnic group, will visit the villages known to harbor significant numbers of A00 members, sample there, and collect information on the families. How do we know which villages have A00?  Because Matthew collected the original Mbo samples, and over 2000 other DNA samples from all over Cameroon, as part of his dissertation research! His data indicate that the Mbo and Bangwa are only two of a number of peoples who have A00 among them. About a dozen other ethnic groups include A00 members, including some Pygmies! Those samples, though, are no longer available to us.

Now it's time to gather our own samples. We have a series of five field trips planned, to gather samples of diverse peoples in Western, Southern and Eastern Cameroon. Our analysis will include some special areas of knowledge from Matthew's studies, such as how different peoples support themselves within forest and grasslands ecologies, and the effects of polygamy vs. monogamy in patterns of populations' Y-chromosome DNA.

In the past, it has always been thought necessary to make DNA donors anonymous when they participate in scientific studies. In this project, however, we'll be asking for the donors' names, for several reasons:
1. We want to give them the possibility of receiving their test results, if they are interested
2. We want there to be a future possibility of families who match them, such as African Americans, to know their matches, if they opt in
3. We hope to gather a second sample (saliva) from one or more donors, in order to have a full Y genome sequence done
4. We hope to correlate the haplogroups and haplotypes we find with families of different known histories, such as royal lineages, traditional religious office-holders, and those that are known to have had ancestors held as slaves by local rulers.

Of course, their names will not be made public except, should they decide to participate and future funding allows it, to their individual DNA matches.

This is a kind of research, combining genealogy with population genetics, that academics rarely undertake, but which has been occasionally done in papers such as this one by one of the co-authors of our last paper, Dr. Krishna Veeramah: Sex-Specific Genetic Data Support One of Two Alternative Versions of the Foundation of the Ruling Dynasty of the Nso in Cameroon.

We have four weeks to raise the $2500 needed to launch our first field trip in Cameroon. Our deadline is August 19th. Then Matthew will set out for the remote mountain villages where he was raised. We look forward to bringing you all along on this great adventure.

In addition, apart from the appeal for fieldwork support per se, we're looking for a few generous individuals who'll help us obtain a decent (can be used) laptop and a digital camera for Matthew, who's a very low-income grad student. We're also looking for a trustworthy person flying to Cameroon who can take these along, saving us the exorbitant shipping fees.

Please write to me if you have any leads.

In the near future, the next fundraising campaign will ask for your support for the DNA extraction and the screening of our first set of samples for A00. Stay tuned! Please visit and "like" our page on Facebook:

https://www.facebook.com/A00.Cameroon.Project

Looking forward to seeing you, with gratitude for your support,

Bonnie Schrack

I hope that some of you will be inspired to help this very worthwhile project. If you want to get in touch with Bonnie you can contact her via the Haplogroup A Project website. You can also find her on Twitter where she goes by the name of @Greenleafy.